Effects of Wuqinxi on Balance, Walking and Quality of Life for Patients with Parkinson's Disease / 中国康复理论与实践

Objective:To observe the effects of traditional Chinese exercise Wuqinxi on balance, walking and quality of life for patients with Parkinson's disease. Methods:From December, 2018 to December, 2020, 62 inpatients with Parkinson's disease in the Affiliated Hospital of Shandong University of Chinese Medicine were randomly divided into control group (n = 31) and observation group (n = 31). The control group received routine medicine and rehabilitation training, while the observation group received Wuqinxi in addition, for eight weeks. The trajectory length and ellipse area of the center of pressure in 30 seconds were measured with PRO-KIN before and after treatment, while they were assessed with Berg Balance Scale (BBS), Timed 'Up and Go' Test (TUGT) and Parkinson's Disease Quality of Life Scale-39 (PDQ-39). Results:The trajectory length and ellipse area of the center of pressure, BBS score, TUGT time, and PDQ-39 score improved in both groups after treatment (t > 11.225, P < 0.001), and all improved more in the observation group than in the control group (t > 5.919, P < 0.001). Conclusions:Wuqinxi training is effective on balance, walking and quality of life for patients with Parkinson's disease.

Effects of Daoyin on Motor Function, Balance and Quality of Life for Patients with Parkinson's Disease / 中国康复理论与实践

Objective:To observe the clinical efficacy of Daoyin on motor function, fall prevention and quality of life in patients with Parkinson's disease (PD). Methods:From August, 2016 to September, 2018, 62 PD patients were randomly divided into control group (n = 31) and observed group (n = 31). The control group received routine medicine and health education, and the observed group received Daoyin exercise in addition, for six weeks. They were assessed with Unified Parkinson's Disease Rating Scale Ⅲ (UPDRSⅢ), Timed "Up and Go" Test (TUGT), modified Falls Efficacy Scale (MFES) and Parkinson's Disease Questionnaire-39 (PDQ-39). Their anteroposterior standard deviation (AP-SD) and mediolateral standard deviation (ML-SD) of centre of pressure were measured with Balancer before and after treatment. Results:The scores of UPDRSⅢ, TUGT, MFES and PDQ-39 improved in the observed group (|t| > 2.463, P < 0.05), as well as the length of AP-SD and ML-SD, and improved more in the observed group than in the control group (|t| > 2.261, P < 0.05) after treatment. Conclusion:Daoyin can obviously improve motor function, balance and quality of life for PD patients, and prevent them from falling.

Valve replacement in pediatric patients: a single center experience / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Reconstructive surgery is the primary goal in pediatric patients with valve disease. However, in cases with irreparable valve lesions, valve replacement is the only option. This study aimed to retrospectively analyze the clinical experience of heart valve prosthesis replacement in children.</p><p><b>METHODS</b>Between January 1990 and July 2009, 35 pediatric patients (16 boys, 19 girls) underwent mechanical valve replacement in Shandong University Qilu Hospital. The ages ranged from 2.5 to 14 years (mean, (8.8 ± 3.8) years) and body weight varied from 11 to 37 kg (mean, (22.1 ± 5.2) kg). Mechanical valve replacement was performed because of congenital heart disease in 23 patients, rheumatic disease in ten patients and infective endocarditis in two patients. St. Jude bileaflet mechanical valves were implanted in all the 35 patients including mitral valve replacement (MVR) in 18, aortic valve replacement (AVR) in 12, tricuspid valve replacement (TVR) in two, AVR and MVR in two and MVR and TVR in one. The size of the prostheses ranged between 19 and 27 mm. All patients received long-term anticoagulation treatment with sodium warfarin, aiming to maintain an international normalized ratio between 1.5 to 2.0. Follow-up was performed in all the patients with a total follow-up of 119.4 patient-years.</p><p><b>RESULTS</b>The operative mortality was 8.57% (3/35). One patient, who underwent cardiac debridement and AVR, died 2 hours after being admitted to the intensive care unit because of severe low cardiac output syndrome and ventricular fibrillation. Two patients died of cardiogenic shock and renal failure during initial hospitalization after the operation. One patient who received replacement of a tricuspid valve developed complete heart block requiring temporary pacing and recovered sinus rhythm 4 days later. Thirty-two patients survived and their cardiac function was in New York Heart Association (NYHA) class I to class II when discharged. Late events included hemorrhage and endocarditis. Two patients required reoperation. No late deaths occurred during the follow-up.</p><p><b>CONCLUSIONS</b>Mechanical valve replacement remains an acceptable treatment option in children when the valve reparation is impossible or unsuccessful. The operative mortality and incidence of any valve-related events such as endocarditis, reoperation, thromboembolism or anticoagulation-related bleeding are acceptable.</p>

Glypican3 in genetically modified human monocyte-derived dendritic cells induced specific cytotoxity against glypican3 overexpressing human hepatocellular carcinoma cells in vitro

This study was performed in China Medical University Shenyang, China from September 2007-February 2008. The design of the study was to modify DCs with GPC3 and to be used to activate human T cells and elicit a cell-mediated immune response against HepG2 in vitro. The GPC3 gene expression was identified by western blot and immunocytochemistry. The proliferation of responder cells and cytotoxicity against HepG2 were examined by water-soluble tetrazolium salt -1 and lactate dehydrogenase assay respectively. The interferon-y [IFN-gamma] secreted was detected by ELISA assay. Both Western blot and immunocytochemical analysis assured the validity of GPC3 transfection. Glypican3 modified DCs were potent in inducing responder cells proliferation and IFN-gamma production. The cytotoxicity in the group of GPC3 transfected DCs were [38.90 +/- 0.95%] at the ratio of effector cells/target cells E/T:100:1, 30.83 +/- 1.24% at the ratio of E/T:50:1, and 23.84 +/- 0.65% at the ratio of E/T:10:1, respectively [which is significant compared with other groups, p<0.001]. And the GPC3 modified DCs showed ability to induce high specific cytotoxicity against HepG2 in vitro. The effector cells stimulated with DCs that were transfected with pEF-hGPC3 plasmid could effectively lyse GPC3 expressing HepG2 cells, which suggested that those genetically engineered DCs have the potential to serve as novel vaccine for HCC

Effects of interleukin-1beta on nitric oxide production and ATP synthesis in rat surplus hepatocytes after partial hepatectomy / 中国医学科学院学报

<p><b>OBJECTIVE</b>To detect the nitric oxide (NO) production and energy metabolism of the interleukin (IL)-1beta-treated residual hepatocytes from rats after partial hepatectomy.</p><p><b>METHODS</b>Forty rats were equally divided into partial hepatectomies (PH) group and control group. In the control group the rats were otherwise matched and underwent sham surgeries. The residual hepatocytes were separated by the collagenase perfusion method. The hepatocytes were cultured with cytokines such as IL-1beta. The production of NO in the two groups were measured with Griess reagent method, the production of inducible nitric oxide synthase (iNOS) protein detected with Western blot, the content of the nucleotide in the hepatocytes detected with high-performance liquid chromatography, and the content of the ketone body in the hepatocytes of the two groups determined with the enzymatic method. Afterwards the ketone body ratio (acetoacetate/beta-hydroxy butyrate, KBR) was calculated.</p><p><b>RESULTS</b>The production of NO in the PH group was twice as much as that in the Sham group. IL-1beta decreased the content of ATP and the KBR in the hepatocytes of both groups, and the decrease magni tude in the PH group was significantly larger than that in the Sham group. After the injection of L-arginine, the production of NO in the hepatocytes in the PH group increased, and the level of ATP and KBR decreased. N(G)-methyl-L-arginine (L-NMMA), the inhibitor of NO synthase, inhibited the production of NO and reversed the decrease of ATP and KBR.</p><p><b>CONCLUSION</b>After partial hepatectomy, increased NO production in the hepatocytes after the treatment of interleukin-1beta may disturb the function of mitochondria by inhibiting the synthesis of ATP.</p>